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KMID : 0363320120330040405
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Journal of Korean Oriental Internal Medicine
2012 Volume.33 No. 4 p.405 ~ p.417
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Effect of Lindera obtusiloba extract on cancer metastasis
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Yun Hyuk
Lee Yong-Jae
Seo Hyun-Won
Park Kyoung-Jae
Ko Ha-Neul
Cha Dong-Seok
Kwon Jin
Jeon Hoon
Kim Kang-San
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Abstract
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Objectives : In the present study, anti-metastatic properties of the methanol extract of L. obtusiloba (MLO) were
evaluated.
Methods : To determine the effect of MLO on cancer metastasis, we checked matrix metalloproteinase-2 (MMP-2) and
matrix metalloproteinase-9 (MMP-9) activities and expressions in B16F10 melanoma cells. In addition, we performed cell
migration assay as well as invasion assay using Matrigel. Finally, we used an in vivo lung metastasis model to confirm the anti-metastatic activity of MLO.
Results : 1. MLO showed potent inhibitory effects on MMP-2 and MMP-9 activities and expressions via down-regulation of
activation of NF-κB in B16F10 melanoma cells. 2. Melanoma cell migration and invasion were down-regulated by MLO treatment. 3. Not only in vitro model, but MLO also significantly suppressed lung metastasis in vivo.
Conclusions : The present results indicate that MLO has strong inhibitory effect on cancer metastasis. Therefore, L.
obtusiloba could be a valuable anti-metastatic agent.
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KEYWORD
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Lindera obtusiloba, matrix metalloproteinase (MMP), cancer metastasis
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